Healthy Bones Co. Collagen Peptides Amino Acid Profile
TECHNICAL INFORMATION
Typical Amino Acid Composition of
Healthy Bones Co. Collagen Peptides (Hydrolysate)*
g/100g**
Amino Acid/100 Amino Acid
ALANINE
8.6
10.7
ARGININE
7.3
4.5
ASPARTIC ACID
5.8
4.8
GLUTAMIC ACID
10.2
7.7
GLYCINE
22.2
32.6
HISTIDINE
1.0
0.6
HYDROXYPROLINE
11.9
10.0
ISOLEUCINE
1.4
1.1
LEUCINE
2.7
2.3
METHIONINE
0.9
0.7
PHENYLALANINE
2.1
1.4
PROLINE
12.7
12.1
SERINE
3.2
3.4
THREONINE
1.8
1.7
LYSINE
3.6
2.6
HYDROXYLYSINE
1.6
1.1
TYROSINE
0.8
0.4
VALINE
2.4
2.3
* Including the following branded products: FORTIBONE®, FORTIGEL®, VERISOL®, TENDOFORTE®
** g amino acid per 100 g crude protein
Method
The amino acid composition was determined by amino acid analysis as described in Pharm. Eu. 2.2.56 (Version 8). The proteins were hydrolysed for 24h to their individual amino acid constituents in the presence of 6 n HCl and 0.1% phenol at 110°C. The amide links in the side chains of glutamine and asparagine are hydrolyzed to form glutamic acid and aspartic acid. Following the hydrolysis, the amino acids are covalently labelled with 6-aminoquinolyl-N -hydroxysuccinimidyl carbamate (AQC; AccQ-Flour reagent, Waters Inc.) using a precolumn derivatisation technique. L-2 Aminobutyric acid (AAbA) with a final concentration of 10 pmol/μl was used as internal standard. The derivatives are separated by C18 reversed-phase HPLC and quantified by fluorescence detection. (Determination of data PROTAGEN AG, Dortmund, Germany).
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BG/2015-10-20